rabbit polyclonal antibodies against bdnf  (Santa Cruz Biotechnology)

Journal: Brain Sciences

Article Title: Neonatal Handling Positively Modulates Anxiety, Sensorimotor Gating, Working Memory, and Cortico-Hippocampal Neuroplastic Adaptations in Two Genetically Selected Rat Strains Differing in Emotional and Cognitive Traits

doi: 10.3390/brainsci15080776

Figure Lengend Snippet: Western blot analysis of the BDNF ( A , B ), trkB ( C , D ), and the PSA-NCAM ( E , F ), in the prefrontal (prelimbic/infralimbic) cortex of the RHA and the RLA rats, either untreated controls (CTRL) or treated with neonatal handling (NH). The values represent the densitometric analysis of the BDNF/GAPDH ( B ), trkB/GAPDH ( D ), and the PSA-NCAM/GAPDH ( F ) band grey optical density (O.D.) ratios. The bars denote the mean ± S.E.M. of 9–10 rats, in each experimental group. ***: p < 0.001 (post hoc Duncan’s multiple range test).

Article Snippet: Primary antibodies included rabbit polyclonal antibodies against BDNF [Cat# N-20 sc-546,RRID:AB_63094, Santa Cruz Biotechnology, Heidelberg, Germany] and trkB [Cat# (794) sc-12,RRID:AB_632557, Santa Cruz Biotechnology], both diluted 1:1000, along with a mouse monoclonal antibody against PSA-NCAM (Cat# MAB5324, RRID:AB_95211, Merck Millipore, Darmstadt, Germany) also diluted 1:1000 in TBS containing 5% milk powder and 0.02% sodium azide.

Techniques: Western Blot

Journal: Brain Sciences

Article Title: Neonatal Handling Positively Modulates Anxiety, Sensorimotor Gating, Working Memory, and Cortico-Hippocampal Neuroplastic Adaptations in Two Genetically Selected Rat Strains Differing in Emotional and Cognitive Traits

doi: 10.3390/brainsci15080776

Figure Lengend Snippet: Western blot analysis of the BDNF ( A , B ), trkB ( C , D ), and the PSA-NCAM ( E , F ), in the anterior cingulate cortex of the RHA and the RLA rats, either untreated controls (CTRL) or treated with neonatal handling (NH). The values represent the densitometric analysis of the BDNF/GAPDH ( B ), trkB/GAPDH ( D ), and the PSA-NCAM/GAPDH ( F ) band grey optical density (O.D.) ratios. The bars denote the mean ± S.E.M. of 9–10 rats, in each experimental group. **: p < 0.01 (post hoc Duncan’s multiple range test).

Article Snippet: Primary antibodies included rabbit polyclonal antibodies against BDNF [Cat# N-20 sc-546,RRID:AB_63094, Santa Cruz Biotechnology, Heidelberg, Germany] and trkB [Cat# (794) sc-12,RRID:AB_632557, Santa Cruz Biotechnology], both diluted 1:1000, along with a mouse monoclonal antibody against PSA-NCAM (Cat# MAB5324, RRID:AB_95211, Merck Millipore, Darmstadt, Germany) also diluted 1:1000 in TBS containing 5% milk powder and 0.02% sodium azide.

Techniques: Western Blot

Journal: Brain Sciences

Article Title: Neonatal Handling Positively Modulates Anxiety, Sensorimotor Gating, Working Memory, and Cortico-Hippocampal Neuroplastic Adaptations in Two Genetically Selected Rat Strains Differing in Emotional and Cognitive Traits

doi: 10.3390/brainsci15080776

Figure Lengend Snippet: Western blot analysis of the BDNF ( A , B ), trkB ( C , D ), and the PSA-NCAM ( E , F ), in the ventral hippocampus of the RHA and the RLA rats, either untreated controls (CTRL) or treated with neonatal handling (NH). The values represent the densitometric analysis of the BDNF/GAPDH ( B ), trkB/GAPDH ( D ), and the PSA-NCAM/GAPDH ( F ) band grey optical density (O.D.) ratios. The bars denote the mean ± S.E.M. of 9–10 rats, in each experimental group. *: p < 0.05; **: p < 0.02 (post hoc Duncan’s multiple range test).

Article Snippet: Primary antibodies included rabbit polyclonal antibodies against BDNF [Cat# N-20 sc-546,RRID:AB_63094, Santa Cruz Biotechnology, Heidelberg, Germany] and trkB [Cat# (794) sc-12,RRID:AB_632557, Santa Cruz Biotechnology], both diluted 1:1000, along with a mouse monoclonal antibody against PSA-NCAM (Cat# MAB5324, RRID:AB_95211, Merck Millipore, Darmstadt, Germany) also diluted 1:1000 in TBS containing 5% milk powder and 0.02% sodium azide.

Techniques: Western Blot

Journal: Brain Sciences

Article Title: Neonatal Handling Positively Modulates Anxiety, Sensorimotor Gating, Working Memory, and Cortico-Hippocampal Neuroplastic Adaptations in Two Genetically Selected Rat Strains Differing in Emotional and Cognitive Traits

doi: 10.3390/brainsci15080776

Figure Lengend Snippet: Western blot analysis of the BDNF ( A , B ), trkB ( C , D ), and the PSA-NCAM ( E , F ), in the dorsal hippocampus of the RHA and the RLA rats, either untreated controls (CTRL) or treated with neonatal handling (NH). The values represent the densitometric analysis of the BDNF/GAPDH ( B ), trkB/GAPDH ( D ), and the PSA-NCAM/GAPDH ( F ) band grey optical density (O.D.) ratios. The bars denote the mean ± S.E.M. of 9–10 rats, in each experimental group.

Article Snippet: Primary antibodies included rabbit polyclonal antibodies against BDNF [Cat# N-20 sc-546,RRID:AB_63094, Santa Cruz Biotechnology, Heidelberg, Germany] and trkB [Cat# (794) sc-12,RRID:AB_632557, Santa Cruz Biotechnology], both diluted 1:1000, along with a mouse monoclonal antibody against PSA-NCAM (Cat# MAB5324, RRID:AB_95211, Merck Millipore, Darmstadt, Germany) also diluted 1:1000 in TBS containing 5% milk powder and 0.02% sodium azide.

Techniques: Western Blot

Journal: Animals : an Open Access Journal from MDPI

Article Title: Immunolocalization of Two Neurotrophins, NGF and BDNF, in the Pancreas of the South American Sea Lion Otaria flavescens and Bottlenose Dolphin Tursiops truncatus

doi: 10.3390/ani14162336

Figure Lengend Snippet: BDNF immunoreactivity in the pancreas of Otaria flavescens and Tursiops truncatus . ( A – D ) Otaria flavescens . BDNF immunorectivity of exocrine cells in parechima ( A ); positive large islet of Langerhans ( B ); positive medium islet of Langerhans ( C ); positive fibers in the wall of blood vessels (arrow), positive neurons (asterisk) and fibers (arrowhead) in the parenchyma ( D ); ( E – H ) Tursiops truncatus. Immunoreactivity of exocrine cells in parenchyma ( E ); positive islet of Langerhans ( F ); positive fibers in the wall of blood vessels (arrow) ( G ); immunoreactive fibers (arrowhead for fiber bundle) ( H ). Scale bar 100 µm.

Article Snippet: Then, sections were incubated overnight at 4 °C with primary antisera, respectively, rabbit polyclonal antibody against NGF diluted at 1:200 (H-20, sc-548 Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and rabbit polyclonal antibody against BDNF diluted at 1:200 (N-20, sc-546 Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) [ ].

Techniques: